PrimePCR™ SYBR® Green Assay: Cuzd1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0016266
Assay Design:   intron_spanning
Chromosome Location:   1:208920103-208921312question
Amplicon Length:   80
Splice Variants Targeted:   ENSRNOT00000050519

Gene Information

protein specifically expressed in uterus and ovary and regulated by estrogen; may function in pregnancy [RGD, Feb 2006]

Gene Symbol:   Cuzd1
Gene Name:   CUB and zona pellucida-like domain-containing protein 1 precursor
Aliases:   Not Available
RefSeq:   NM_054005
Ensembl:   ENSRNOG00000029945
Entrez:   117179
UniGene:   Rn.10825
Chromosome Mapping:   1q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.530000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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