PrimePCR™ SYBR® Green Assay: Pdcd1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Pdcd1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Pdcd1, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0014083
Assay Design:   intron_spanning
Chromosome Location:   9:100940163-100950159question
Amplicon Length:   118
Splice Variants Targeted:   ENSRNOT00000072377 ENSRNOT00000031704 ENSRNOT00000000514 ENSRNOT00000015977 ENSRNOT00000068077 ENSRNOT00000067389 ENSRNOT00000067774 ENSRNOT00000025743 ENSRNOT00000074915 ENSRNOT00000021840 ENSRNOT00000067679

Gene Information

Description Not Available

Gene Symbol:   Pdcd1
Gene Name:   Programmed cell death 1 (Predicted); Protein Pdcd1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049797
Entrez:   301626
Chromosome Mapping:   9q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.120000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download