PrimePCR™ SYBR® Green Assay: Lsm6, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0011380
Assay Design:   intron_spanning
Chromosome Location:   13:52469322-52471353question
Amplicon Length:   104
Splice Variants Targeted:   ENSRNOT00000035345 ENSRNOT00000035303 ENSRNOT00000009332 ENSRNOT00000071471 ENSRNOT00000050289 ENSRNOT00000008936

Gene Information

Description Not Available

Gene Symbol:   Lsm6
Gene Name:   Protein Lsm6; RCG51654, isoform CRA_a; RCG63582, isoform CRA_b
Aliases:   Not Available
RefSeq:   NM_001126085
Ensembl:   ENSRNOG00000030374
Entrez:   498934
UniGene:   Rn.139376
Chromosome Mapping:   19q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.320000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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