PrimePCR™ SYBR® Green Assay: Prss2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Prss2, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Prss2, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0010973
Assay Design:   intron_spanning
Chromosome Location:   4:135417187-135418546question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000018042 ENSRNOT00000041100 ENSRNOT00000066789 ENSRNOT00000048049 ENSRNOT00000018852 ENSRNOT00000029319 ENSRNOT00000044960 ENSRNOT00000043527 ENSRNOT00000040403

Gene Information

catalyzes the cleavage of Arg-Xaa, Lys-Xaa [RGD, Feb 2006]

Gene Symbol:   Prss2
Gene Name:   Anionic trypsin-2 precursor
Aliases:   Not Available
RefSeq:   NM_012729
Ensembl:   ENSRNOG00000049576
Entrez:   25052
Chromosome Mapping:   4q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.950000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download