PrimePCR™ SYBR® Green Assay: Rpl35a, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Rpl35a, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Rpl35a, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0009987
Assay Design:   intron_spanning
Chromosome Location:   11:74049740-74051149question
Amplicon Length:   87
Splice Variants Targeted:   ENSRNOT00000041291 ENSRNOT00000050438 ENSRNOT00000050968 ENSRNOT00000059047 ENSRNOT00000043587 ENSRNOT00000043508 ENSRNOT00000042285 ENSRNOT00000043796 ENSRNOT00000040261 ENSRNOT00000049614

Gene Information

ribosomal protein that binds tRNA [RGD, Feb 2006]

Gene Symbol:   Rpl35a
Gene Name:   60S ribosomal protein L35a
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000038678
Entrez:   57809
Chromosome Mapping:   12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.730000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download