PrimePCR™ SYBR® Green Assay: Acly, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0009633
Assay Design:   intron_spanning
Chromosome Location:   10:88201753-88206339question
Amplicon Length:   142
Splice Variants Targeted:   ENSRNOT00000064861 ENSRNOT00000034759 ENSRNOT00000023447 ENSRNOT00000043172

Gene Information

catalyzes the synthesis of cytosolic acetyl-CoA; plays a role in lipid and cholesterol metabolism [RGD, Feb 2006]

Gene Symbol:   Acly
Gene Name:   ATP-citrate synthase isoform 1
Aliases:   Not Available
RefSeq:   NM_001111095 NM_016987
Ensembl:   ENSRNOG00000016924
Entrez:   24159
UniGene:   Rn.29771
Chromosome Mapping:   10q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.720000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download