PrimePCR™ SYBR® Green Assay: Tsg101, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0009591
Assay Design:   intron_spanning
Chromosome Location:   1:104031459-104033328question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000018194

Gene Information

forms a transactivation complex with apoptosis-antagonizing transcription factor (AATF); regulates monoubiquitination of the androgen receptor [RGD, Feb 2006]

Gene Symbol:   Tsg101
Gene Name:   Tumor susceptibility gene 101 protein
Aliases:   Not Available
RefSeq:   NM_181628
Ensembl:   ENSRNOG00000013381
Entrez:   292925
UniGene:   Rn.7410
Chromosome Mapping:   1q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.070000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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