PrimePCR™ SYBR® Green Assay: Esr1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0009588
Assay Design:   intron_spanning
Chromosome Location:   1:42873088-42901908question
Amplicon Length:   103
Splice Variants Targeted:   ENSRNOT00000026350

Gene Information

acts as a transcriptional activator when bound to estrogen; may play a role in myocardial regulation [RGD, Feb 2006]

Gene Symbol:   Esr1
Gene Name:   Estrogen receptor
Aliases:   Not Available
RefSeq:   NM_012689
Ensembl:   ENSRNOG00000019358
Entrez:   24890
UniGene:   Rn.10595,Rn.231229
Chromosome Mapping:   1q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 37.910000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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