PrimePCR™ SYBR® Green Assay: Tac1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Tac1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Tac1, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0007480
Assay Design:   intron_spanning
Chromosome Location:   4:33500483-33501562question
Amplicon Length:   111
Splice Variants Targeted:   ENSRNOT00000009888 ENSRNOT00000034719 ENSRNOT00000052333 ENSRNOT00000016652 ENSRNOT00000005100

Gene Information

encodes neuropeptides substance P, neurokinin A, neuropeptide K, and neuropeptide gamma [RGD, Feb 2006]

Gene Symbol:   Tac1
Gene Name:   Protachykinin-1 Substance P Neurokinin A Neuropeptide K Neuropeptide gamma C-terminal-flanking peptide
Aliases:   Not Available
RefSeq:   NM_012666 NM_001124770 NM_001124768 NM_001124769
Ensembl:   ENSRNOG00000007374
Entrez:   24806
Chromosome Mapping:   4q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.620000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download