PrimePCR™ SYBR® Green Assay: Tgfbr2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0006991
Assay Design:   intron_spanning
Chromosome Location:   8:123600920-123604690question
Amplicon Length:   101
Splice Variants Targeted:   ENSRNOT00000037883

Gene Information

receptor for TGF-beta; may play a role in lung maturation and response to hypoxia [RGD, Feb 2006]

Gene Symbol:   Tgfbr2
Gene Name:   TGF-beta receptor type-2
Aliases:   Not Available
RefSeq:   NM_031132
Ensembl:   ENSRNOG00000013265
Entrez:   81810
UniGene:   Rn.9954
Chromosome Mapping:   8q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.500000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download