This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: Mapk9, Rat
PrimePCR™ Template for SYBR® Green Assay: Mapk9, Rat
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases are multifunctional proteins. They are involved in a wide variety of cellular processes such as growth, proliferation, differentiation, transcription regulation, and development. They function in stress responses, apoptosis, inflammation and transformation. For example, this kinase plays a dominant role in mediating proliferation of lung cancer and prostate cancer cells, and in regulation of osmotic stress-induced tight junction disruption. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.