PrimePCR™ SYBR® Green Assay: Masp2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0006171
Assay Design:   intron_spanning
Chromosome Location:   5:169084488-169085760question
Amplicon Length:   110
Splice Variants Targeted:   ENSRNOT00000016317

Gene Information

member of the MBL/MASPs/Map19 complex of the mannan binding lectin pathway of complement activation; catalyzes the cleavage of complement component C4 [RGD, Feb 2006]

Gene Symbol:   Masp2
Gene Name:   Mannan-binding lectin serine protease 2 precursor
Aliases:   Not Available
RefSeq:   NM_172043
Ensembl:   ENSRNOG00000011258
Entrez:   64459
UniGene:   Rn.45144
Chromosome Mapping:   5q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.070000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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