PrimePCR™ SYBR® Green Assay: Shc1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0005836
Assay Design:   intron_spanning
Chromosome Location:   2:208163284-208164503question
Amplicon Length:   147
Splice Variants Targeted:   ENSRNOT00000022014 ENSRNOT00000056652 ENSRNOT00000028038

Gene Information

mouse homolog interacts with growth factor receptors; involved in transformation and differentiation in a Ras-dependent fashion [RGD, Feb 2006]

Gene Symbol:   Shc1
Gene Name:   SHC-transforming protein 1 isoform a
Aliases:   Not Available
RefSeq:   NM_053517 NM_001164060
Ensembl:   ENSRNOG00000020657
Entrez:   85385
UniGene:   Rn.138818
Chromosome Mapping:   2q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.100000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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