PrimePCR™ SYBR® Green Assay: Rhebl1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0005228
Assay Design:   intron_spanning
Chromosome Location:   X:115063798-115064878question
Amplicon Length:   114
Splice Variants Targeted:   ENSRNOT00000035659

Gene Information

has similarity to the Rab subfamily of GTPases [RGD, Feb 2006]

Gene Symbol:   Rhebl1
Gene Name:   GTPase RhebL1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000021571
Entrez:   359959
UniGene:   Rn.43211
Chromosome Mapping:   X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.840000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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