PrimePCR™ SYBR® Green Assay: Wnk1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Wnk1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Wnk1, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0004991
Assay Design:   intron_spanning
Chromosome Location:   4:219550145-219551088question
Amplicon Length:   98
Splice Variants Targeted:   ENSRNOT00000013355 ENSRNOT00000013621 ENSRNOT00000005422 ENSRNOT00000019370

Gene Information

catalyzes the phosphorylation of myelin basic protein in vitro; has autophosphorylation activity; may regulate the thiazide-sensitive Na-Cl cotransporter NCC [RGD, Feb 2006]

Gene Symbol:   Wnk1
Gene Name:   Serine/threonine-protein kinase WNK1
Aliases:   Not Available
RefSeq:   NM_053794 NM_001002823 NM_001199095
Ensembl:   ENSRNOG00000009956
Entrez:   116477
Chromosome Mapping:   4q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.410000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download