PrimePCR™ SYBR® Green Assay: Tcf3, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0004852
Assay Design:   intron_spanning
Chromosome Location:   7:12331958-12334389question
Amplicon Length:   199
Splice Variants Targeted:   ENSRNOT00000025284 ENSRNOT00000023473 ENSRNOT00000023597 ENSRNOT00000050010

Gene Information

transcriptional regulator; binds enhancer motifs of pancreatic exocrine genes [RGD, Feb 2006]

Gene Symbol:   Tcf3
Gene Name:   Transcription factor E2-alpha isoform b
Aliases:   Not Available
RefSeq:   NM_133524 NM_001035237
Ensembl:   ENSRNOG00000016970
Entrez:   171046
UniGene:   Rn.10290
Chromosome Mapping:   7q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.890000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download