PrimePCR™ SYBR® Green Assay: Col11a1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0004654
Assay Design:   intron_spanning
Chromosome Location:   2:235030200-235034097question
Amplicon Length:   106
Splice Variants Targeted:   ENSRNOT00000074359 ENSRNOT00000061032 ENSRNOT00000074941 ENSRNOT00000023794 ENSRNOT00000068413 ENSRNOT00000024138 ENSRNOT00000023693 ENSRNOT00000016273

Gene Information

subunit of heterotrimeric collagen XI molecule which is expressed in developing cartilage and plays a role in regulation of collagen fibril diameter size; alternatively spliced as six different isoforms; may be a substrate for BMP-1 [RGD, Feb 2006]

Gene Symbol:   Col11a1
Gene Name:   Collagen alpha-1(XI) chain
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000023148
Entrez:   25654
Chromosome Mapping:   2q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.790000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download