PrimePCR™ SYBR® Green Assay: Trmt11, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0003642
Assay Design:   intron_spanning
Chromosome Location:   1:30911174-30926772question
Amplicon Length:   108
Splice Variants Targeted:   ENSRNOT00000019436

Gene Information

putatuive member of the RNA methylase enzyme family [RGD, Feb 2006]

Gene Symbol:   Trmt11
Gene Name:   TRNA (guanine(10)-N2)-methyltransferase homolog
Aliases:   Not Available
RefSeq:   NM_198051
Ensembl:   ENSRNOG00000014240
Entrez:   378794
UniGene:   Rn.214011
Chromosome Mapping:   1p11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.970000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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