PrimePCR™ SYBR® Green Assay: Lat2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0003616
Assay Design:   intron_spanning
Chromosome Location:   12:27120464-27122618question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000034629

Gene Information

human homolog maps to the microdeletion region in Williams syndrome and may act as an adaptor for non T-cell immune response [RGD, Feb 2006]

Gene Symbol:   Lat2
Gene Name:   Linker for activation of T-cells family member 2 precursor
Aliases:   Not Available
RefSeq:   NM_173840
Ensembl:   ENSRNOG00000021856
Entrez:   317676
UniGene:   Rn.163206
Chromosome Mapping:   12q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.750000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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