PrimePCR™ SYBR® Green Assay: Syn2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0003176
Assay Design:   intron_spanning
Chromosome Location:   4:210461729-210463486question
Amplicon Length:   108
Splice Variants Targeted:   ENSRNOT00000011292

Gene Information

neuronal phosphoprotein that coats synaptic vesicles; binds to the cytoskeleton; may be important for vesicle trafficking to synapses and for the regulation of neurotransmitter release [RGD, Feb 2006]

Gene Symbol:   Syn2
Gene Name:   Synapsin-2 isoform 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000008157
Entrez:   29179
UniGene:   Rn.506
Chromosome Mapping:   4q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.560000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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