PrimePCR™ SYBR® Green Assay: Gucy1b2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0002663
Assay Design:   intron_spanning
Chromosome Location:   15:49300191-49311666question
Amplicon Length:   107
Splice Variants Targeted:   ENSRNOT00000012948

Gene Information

subunit of soluble guanylate cyclase, which catalyzes the conversion of GTP to cyclic GMP; may be a candidate gene for involvement in blood pressure regulation [RGD, Feb 2006]

Gene Symbol:   Gucy1b2
Gene Name:   Guanylate cyclase soluble subunit beta-2 isoform 2
Aliases:   Not Available
RefSeq:   NM_012770
Ensembl:   ENSRNOG00000009440
Entrez:   25206
UniGene:   Rn.10933
Chromosome Mapping:   15p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.550000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download