PrimePCR™ SYBR® Green Assay: Acacb, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0002654
Assay Design:   intron_spanning
Chromosome Location:   12:49972096-49975733question
Amplicon Length:   199
Splice Variants Targeted:   ENSRNOT00000001309 ENSRNOT00000067975 ENSRNOT00000000821

Gene Information

catalyzes the carboxylation of acetyl-CoA to malonyl-CoA during fatty acid biosynthesis; involved in fatty acid oxidation and insulin secretion signaling [RGD, Feb 2006]

Gene Symbol:   Acacb
Gene Name:   Acetyl-CoA carboxylase 2
Aliases:   Not Available
RefSeq:   NM_053922
Ensembl:   ENSRNOG00000000658
Entrez:   116719
UniGene:   Rn.162151
Chromosome Mapping:   12q16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.300000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download