PrimePCR™ SYBR® Green Assay: Mthfd1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0002354
Assay Design:   intron_spanning
Chromosome Location:   6:108759934-108761764question
Amplicon Length:   109
Splice Variants Targeted:   ENSRNOT00000008374

Gene Information

a trifunctional enzyme combining a 10-formyl-H4folate synthetase, a 5,10-methenyl-H4folate cyclohydrolase, and a 5,10-methylene-H4folate dehydrogenase [RGD, Feb 2006]

Gene Symbol:   Mthfd1
Gene Name:   C-1-tetrahydrofolate synthase, cytoplasmic
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000005602
Entrez:   64300
UniGene:   Rn.1114
Chromosome Mapping:   6q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.440000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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