This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: Igf2bp2, Rat
PrimePCR™ Template for SYBR® Green Assay: Igf2bp2, Rat
The protein encoded by this gene is a member of a family of mRNA binding proteins that have been shown to bind to several different mRNAs in mammalian cells, including insulin-like growth factor 2 mRNA. Generation of two different isoforms by alternative translation initiation at an internal start codon, has been described for this gene (PMID:22427968). [provided by RefSeq, Jul 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.