PrimePCR™ SYBR® Green Assay: Uso1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0001512
Assay Design:   intron_spanning
Chromosome Location:   14:17326174-17341450question
Amplicon Length:   106
Splice Variants Targeted:   ENSRNOT00000003277

Gene Information

tethering protein involved with transcytotic vesicle transport, including ER to Golgi and between the Golgi stacks [RGD, Feb 2006]

Gene Symbol:   Uso1
Gene Name:   General vesicular transport factor p115
Aliases:   Not Available
RefSeq:   NM_019379
Ensembl:   ENSRNOG00000002301
Entrez:   56042
UniGene:   Rn.4746
Chromosome Mapping:   14p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.940000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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