PrimePCR™ SYBR® Green Assay: Entpd1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0001305
Assay Design:   intron_spanning
Chromosome Location:   1:267254803-267259466question
Amplicon Length:   102
Splice Variants Targeted:   ENSRNOT00000054719

Gene Information

has apyrase activity; may inactivate signaling mediated by extracellular nucleotides [RGD, Feb 2006]

Gene Symbol:   Entpd1
Gene Name:   Ectonucleoside triphosphate diphosphohydrolase 1
Aliases:   Not Available
RefSeq:   NM_022587
Ensembl:   ENSRNOG00000014574
Entrez:   64519
UniGene:   Rn.17491
Chromosome Mapping:   1q54

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.650000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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