PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000016400, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0049616
Assay Design:   Exonic
Chromosome Location:   X:148228680-148228829question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000039927 ENSRNOT00000064632 ENSRNOT00000023364 ENSRNOT00000036706 ENSRNOT00000036427 ENSRNOT00000042763 ENSRNOT00000021269 ENSRNOT00000061340 ENSRNOT00000032824 ENSRNOT00000032908 ENSRNOT00000035594 ENSRNOT00000000455 ENSRNOT00000040427 ENSRNOT00000039932 ENSRNOT00000044035 ENSRNOT00000034533 ENSRNOT00000039910 ENSRNOT00000048148 ENSRNOT00000049734 ENSRNOT00000046064 ENSRNOT00000041811 ENSRNOT00000042656 ENSRNOT00000022122 ENSRNOT00000045544 ENSRNOT00000047868 ENSRNOT00000047948 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000016400
Gene Name:   Glyceraldehyde-3-phosphate dehydrogenase
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000016400
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.540000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download