PrimePCR™ Probe Assay: RGD1563220, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RGD1563220, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RGD1563220, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0048199
Assay Design:   Exonic
Chromosome Location:   15:46923958-46924090question
Amplicon Length:   103
Splice Variants Targeted:   ENSRNOT00000029007 ENSRNOT00000036333 ENSRNOT00000048646 ENSRNOT00000006754 ENSRNOT00000044551 ENSRNOT00000017542 ENSRNOT00000045509 ENSRNOT00000013435 ENSRNOT00000064857 ENSRNOT00000019331 ENSRNOT00000013313 ENSRNOT00000071898 ENSRNOT00000052141 ENSRNOT00000050690 ENSRNOT00000041103 ENSRNOT00000031545 ENSRNOT00000047694 ENSRNOT00000041927 ENSRNOT00000047511 ENSRNOT00000047713 ENSRNOT00000045222 ENSRNOT00000051207 ENSRNOT00000043491 ENSRNOT00000048783

Gene Information

Description Not Available

Gene Symbol:   RGD1563220
Gene Name:   Protein RGD1563220
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000031532
Entrez:   498540
Chromosome Mapping:   15p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 37.310000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download