PrimePCR™ Probe Assay: Prp15, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0048117
Assay Design:   Exonic
Chromosome Location:   4:228751105-228751202question
Amplicon Length:   68
Splice Variants Targeted:   ENSRNOT00000007139 ENSRNOT00000045884

Gene Information

member of a family of salivary proline-rich proteins [RGD, Feb 2006]

Gene Symbol:   Prp15
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   NM_012632
Ensembl:   ENSRNOG00000028983
Entrez:   24687
UniGene:   Rn.9841
Chromosome Mapping:   4q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 37.350000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download