PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000027246, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0048084
Assay Design:   Exonic
Chromosome Location:   4:146954143-146954283question
Amplicon Length:   111
Splice Variants Targeted:   ENSRNOT00000072867 ENSRNOT00000073928 ENSRNOT00000073457 ENSRNOT00000071761 ENSRNOT00000007807 ENSRNOT00000008023 ENSRNOT00000072302 ENSRNOT00000073730 ENSRNOT00000071668 ENSRNOT00000036394 ENSRNOT00000065426 ENSRNOT00000021647 ENSRNOT00000021909 ENSRNOT00000009951

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000027246
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000027246
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 37.360000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download