This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: Pou4f1, Rat
PrimePCR™ Template for Probe Assay: Pou4f1, Rat
transcription factor containing the Pit1-Oct1-Unc86 (POU) domain; regulates expression of Trk receptors and Bcl-2 family members; associated with sensory neuron survival [RGD, Feb 2006]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.