PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000030586, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: No symbol available for ENSRNOG00000030586, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: No symbol available for ENSRNOG00000030586, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0047465
Assay Design:   Exonic
Chromosome Location:   8:90795709-90795858question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000021596 ENSRNOT00000015104 ENSRNOT00000020032 ENSRNOT00000066710 ENSRNOT00000026518 ENSRNOT00000022836 ENSRNOT00000021725 ENSRNOT00000036959 ENSRNOT00000011041 ENSRNOT00000001164 ENSRNOT00000041852 ENSRNOT00000005876 ENSRNOT00000056170 ENSRNOT00000008387 ENSRNOT00000041388 ENSRNOT00000032861 ENSRNOT00000034305 ENSRNOT00000067142 ENSRNOT00000043474 ENSRNOT00000022426 ENSRNOT00000027470 ENSRNOT00000041210 ENSRNOT00000003002 ENSRNOT00000042736

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000030586
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000030586
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.996100
y-intercept 37.960000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download