PrimePCR™ Probe Assay: Wfdc15a, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Wfdc15a, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Wfdc15a, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0047135
Assay Design:   Exonic
Chromosome Location:   3:166776318-166776547question
Amplicon Length:   64
Splice Variants Targeted:   ENSRNOT00000033164

Gene Information

putative member of the WAP-type four disulfide core domain family, a family of protease inhibitors containing the WAP signature motif; human homolog may be a tumor suppressor gene [RGD, Feb 2006]

Gene Symbol:   Wfdc15a
Gene Name:   WAP four-disulfide core domain protein 15A precursor
Aliases:   Not Available
RefSeq:   NM_001008869
Ensembl:   ENSRNOG00000026369
Entrez:   408226
Chromosome Mapping:   3q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 37.640000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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