PrimePCR™ Probe Assay: Gm6712, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Gm6712, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Gm6712, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0046760
Assay Design:   Exonic
Chromosome Location:   18:86670203-86670313question
Amplicon Length:   81
Splice Variants Targeted:   ENSRNOT00000008035 ENSRNOT00000072930 ENSRNOT00000030857 ENSRNOT00000033772 ENSRNOT00000070851 ENSRNOT00000006044 ENSRNOT00000061526 ENSRNOT00000075257 ENSRNOT00000075542 ENSRNOT00000049457 ENSRNOT00000074655 ENSRNOT00000073650 ENSRNOT00000043131 ENSRNOT00000068215 ENSRNOT00000044065 ENSRNOT00000048419 ENSRNOT00000047645

Gene Information

Description Not Available

Gene Symbol:   Gm6712
Gene Name:   Predicted gene 6712
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046450
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.910000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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