PrimePCR™ Probe Assay: Ndufa10, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0046389
Assay Design:   Exonic
Chromosome Location:   9:99282391-99282506question
Amplicon Length:   86
Splice Variants Targeted:   ENSRNOT00000022089

Gene Information

Description Not Available

Gene Symbol:   Ndufa10
Gene Name:   NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
Aliases:   Not Available
RefSeq:   NM_199495
Ensembl:   ENSRNOG00000016470
Entrez:   678759
Chromosome Mapping:   9q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 37.700000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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