PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000042329, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: No symbol available for ENSRNOG00000042329, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: No symbol available for ENSRNOG00000042329, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0044564
Assay Design:   exonic
Chromosome Location:   20:13704460-13704593question
Amplicon Length:   104
Splice Variants Targeted:   ENSRNOT00000001290 ENSRNOT00000034940 ENSRNOT00000072509 ENSRNOT00000008018 ENSRNOT00000022802 ENSRNOT00000066482 ENSRNOT00000001633 ENSRNOT00000068445 ENSRNOT00000060005 ENSRNOT00000065978 ENSRNOT00000005492 ENSRNOT00000005846 ENSRNOT00000010796 ENSRNOT00000065280 ENSRNOT00000014921 ENSRNOT00000018294 ENSRNOT00000039663 ENSRNOT00000027263 ENSRNOT00000005684 ENSRNOT00000032306 ENSRNOT00000023020 ENSRNOT00000022824 ENSRNOT00000073516 ENSRNOT00000071454 ENSRNOT00000064565

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000042329
Gene Name:   Uncharacterized protein LOC689927
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000042329
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.110000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download