PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000025797, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: No symbol available for ENSRNOG00000025797, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: No symbol available for ENSRNOG00000025797, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0043206
Assay Design:   exonic
Chromosome Location:   13:85793846-85793978question
Amplicon Length:   103
Splice Variants Targeted:   ENSRNOT00000039927 ENSRNOT00000064632 ENSRNOT00000023364 ENSRNOT00000021269 ENSRNOT00000003755 ENSRNOT00000008094 ENSRNOT00000035216 ENSRNOT00000039932 ENSRNOT00000034533 ENSRNOT00000049734 ENSRNOT00000049603 ENSRNOT00000037885 ENSRNOT00000047868 ENSRNOT00000050443 ENSRNOT00000026712 ENSRNOT00000051417 ENSRNOT00000073907 ENSRNOT00000047750 ENSRNOT00000058917 ENSRNOT00000041328 ENSRNOT00000034990

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000025797
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000025797
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.830000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download