PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000050264, Rat


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0042709
Assay Design:   exonic
Chromosome Location:   12:13066689-13066816question
Amplicon Length:   98
Splice Variants Targeted:   ENSRNOT00000034681 ENSRNOT00000072406 ENSRNOT00000059247 ENSRNOT00000026730 ENSRNOT00000015504 ENSRNOT00000065693 ENSRNOT00000022244 ENSRNOT00000032631 ENSRNOT00000073167 ENSRNOT00000035638 ENSRNOT00000004965 ENSRNOT00000009205 ENSRNOT00000038120 ENSRNOT00000059892 ENSRNOT00000011239 ENSRNOT00000030319 ENSRNOT00000037571 ENSRNOT00000017454 ENSRNOT00000037416 ENSRNOT00000009436 ENSRNOT00000014849 ENSRNOT00000061630 ENSRNOT00000061754 ENSRNOT00000000474 ENSRNOT00000017684 ENSRNOT00000048888 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000050264
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050264
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.470000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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