PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000042166, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: No symbol available for ENSRNOG00000042166, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: No symbol available for ENSRNOG00000042166, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0042708
Assay Design:   exonic
Chromosome Location:   14:6201394-6201486question
Amplicon Length:   63
Splice Variants Targeted:   ENSRNOT00000035992 ENSRNOT00000030857 ENSRNOT00000033772 ENSRNOT00000042498 ENSRNOT00000047433 ENSRNOT00000043099 ENSRNOT00000031481 ENSRNOT00000070851 ENSRNOT00000073416 ENSRNOT00000008101 ENSRNOT00000008047 ENSRNOT00000075257 ENSRNOT00000042110 ENSRNOT00000075012 ENSRNOT00000075542 ENSRNOT00000072043 ENSRNOT00000049457 ENSRNOT00000051082 ENSRNOT00000074655 ENSRNOT00000072350 ENSRNOT00000073650 ENSRNOT00000043131 ENSRNOT00000075091 ENSRNOT00000049165 ENSRNOT00000068215 ENSRNOT00000047645

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000042166
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000042166
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.070000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download