PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000043155, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0042552
Assay Design:   exonic
Chromosome Location:   3:21833746-21833889question
Amplicon Length:   114
Splice Variants Targeted:   ENSRNOT00000048879 ENSRNOT00000040386 ENSRNOT00000047784 ENSRNOT00000046231 ENSRNOT00000048523 ENSRNOT00000048351 ENSRNOT00000046489 ENSRNOT00000074266 ENSRNOT00000048829 ENSRNOT00000074382 ENSRNOT00000063862 ENSRNOT00000043097 ENSRNOT00000058260 ENSRNOT00000065927 ENSRNOT00000040689 ENSRNOT00000043673 ENSRNOT00000031991 ENSRNOT00000042165 ENSRNOT00000045492 ENSRNOT00000075229 ENSRNOT00000048493

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000043155
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000043155
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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