PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000048426, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0042280
Assay Design:   exonic
Chromosome Location:   20:10679934-10680063question
Amplicon Length:   100
Splice Variants Targeted:   ENSRNOT00000011314 ENSRNOT00000032311 ENSRNOT00000045982 ENSRNOT00000040481 ENSRNOT00000047587 ENSRNOT00000042264 ENSRNOT00000041813 ENSRNOT00000048346 ENSRNOT00000043008 ENSRNOT00000050041 ENSRNOT00000072213

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000048426
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000048426
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.970000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download