PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000021319, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0042201
Assay Design:   exonic
Chromosome Location:   X:55898042-55898170question
Amplicon Length:   99
Splice Variants Targeted:   ENSRNOT00000039927 ENSRNOT00000064632 ENSRNOT00000023364 ENSRNOT00000036427 ENSRNOT00000042763 ENSRNOT00000032406 ENSRNOT00000021269 ENSRNOT00000045607 ENSRNOT00000061340 ENSRNOT00000039569 ENSRNOT00000035566 ENSRNOT00000003755 ENSRNOT00000064584 ENSRNOT00000035594 ENSRNOT00000039932 ENSRNOT00000037429 ENSRNOT00000043042 ENSRNOT00000049734 ENSRNOT00000041811 ENSRNOT00000042656 ENSRNOT00000008851 ENSRNOT00000022122 ENSRNOT00000043243 ENSRNOT00000045544 ENSRNOT00000047868 ENSRNOT00000038429 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000021319
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000021319
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.140000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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