PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000028841, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0041956
Assay Design:   exonic
Chromosome Location:   20:5766816-5766928question
Amplicon Length:   83
Splice Variants Targeted:   ENSRNOT00000002895 ENSRNOT00000067391 ENSRNOT00000025694 ENSRNOT00000003092 ENSRNOT00000000274 ENSRNOT00000031977 ENSRNOT00000049180 ENSRNOT00000015504 ENSRNOT00000013816 ENSRNOT00000019408 ENSRNOT00000068594 ENSRNOT00000048218 ENSRNOT00000074737 ENSRNOT00000032780 ENSRNOT00000040069 ENSRNOT00000001995 ENSRNOT00000067633 ENSRNOT00000004026 ENSRNOT00000005389 ENSRNOT00000001235 ENSRNOT00000050789 ENSRNOT00000035034 ENSRNOT00000010454 ENSRNOT00000056735 ENSRNOT00000057562 ENSRNOT00000011246 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000028841
Gene Name:   Metallothionein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000028841
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 37.300000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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