PrimePCR™ Probe Assay: Ensa, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0041058
Assay Design:   exonic
Chromosome Location:   2:217243144-217243259question
Amplicon Length:   86
Splice Variants Targeted:   ENSRNOT00000047194 ENSRNOT00000025067 ENSRNOT00000014925 ENSRNOT00000033672 ENSRNOT00000018278 ENSRNOT00000075511 ENSRNOT00000040845 ENSRNOT00000023867 ENSRNOT00000064254

Gene Information

may mediate insulin secretion through interaction with the pancreatic beta-cell ATP-sensitive potassium (K(ATP)) channel [RGD, Feb 2006]

Gene Symbol:   Ensa
Gene Name:   Alpha-endosulfine
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000048617
Entrez:   60334
UniGene:   Rn.27154
Chromosome Mapping:   2q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.460000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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