PrimePCR™ Probe Assay: Nphs1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0039793
Assay Design:   exonic
Chromosome Location:   1:90105293-90105412question
Amplicon Length:   90
Splice Variants Targeted:   ENSRNOT00000066186 ENSRNOT00000045212 ENSRNOT00000028358

Gene Information

renal protein localized specifically to glomerular capillary walls; may contribute to the development of proteinuria in renal disease [RGD, Feb 2006]

Gene Symbol:   Nphs1
Gene Name:   Nephrin precursor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000020873
Entrez:   64563
UniGene:   Rn.48745
Chromosome Mapping:   1q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.950000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download