PrimePCR™ Probe Assay: Pigx, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0039055
Assay Design:   exonic
Chromosome Location:   11:75029296-75029397question
Amplicon Length:   72
Splice Variants Targeted:   ENSRNOT00000043365

Gene Information

This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. The protein is translated from a non-AUG translation initiation site. [provided by RefSeq, Jul 2008]

Gene Symbol:   Pigx
Gene Name:   Phosphatidylinositol-glycan biosynthesis class X protein precursor
Aliases:   Not Available
RefSeq:   NM_001100651
Ensembl:   ENSRNOG00000033623
Entrez:   288041
UniGene:   Rn.34037
Chromosome Mapping:   11q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.540000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download