PrimePCR™ Probe Assay: Gm10142, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0038671
Assay Design:   exonic
Chromosome Location:   20:13792676-13792811question
Amplicon Length:   106
Splice Variants Targeted:   ENSRNOT00000001085 ENSRNOT00000007726 ENSRNOT00000046318 ENSRNOT00000048985 ENSRNOT00000019921 ENSRNOT00000073009 ENSRNOT00000060410 ENSRNOT00000001277 ENSRNOT00000006122 ENSRNOT00000073225 ENSRNOT00000065706 ENSRNOT00000038285 ENSRNOT00000010658 ENSRNOT00000052386 ENSRNOT00000026358 ENSRNOT00000007761 ENSRNOT00000067532 ENSRNOT00000056109 ENSRNOT00000015488 ENSRNOT00000001635

Gene Information

Description Not Available

Gene Symbol:   Gm10142
Gene Name:   Predicted gene 10142
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000001220
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.690000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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