PrimePCR™ Probe Assay: Gm10142, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Gm10142, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Gm10142, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0038671
Assay Design:   exonic
Chromosome Location:   20:13792676-13792811question
Amplicon Length:   106
Splice Variants Targeted:   ENSRNOT00000001085 ENSRNOT00000007726 ENSRNOT00000046318 ENSRNOT00000048985 ENSRNOT00000019921 ENSRNOT00000073009 ENSRNOT00000060410 ENSRNOT00000001277 ENSRNOT00000006122 ENSRNOT00000073225 ENSRNOT00000065706 ENSRNOT00000038285 ENSRNOT00000010658 ENSRNOT00000052386 ENSRNOT00000026358 ENSRNOT00000007761 ENSRNOT00000067532 ENSRNOT00000056109 ENSRNOT00000015488 ENSRNOT00000001635

Gene Information

Description Not Available

Gene Symbol:   Gm10142
Gene Name:   Predicted gene 10142
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000001220
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.690000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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