PrimePCR™ Probe Assay: RGD1562953, Rat


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0038521
Assay Design:   exonic
Chromosome Location:   1:289402898-289403060question
Amplicon Length:   133
Splice Variants Targeted:   ENSRNOT00000029007 ENSRNOT00000036333 ENSRNOT00000048646 ENSRNOT00000006485 ENSRNOT00000009816 ENSRNOT00000049436 ENSRNOT00000006754 ENSRNOT00000044551 ENSRNOT00000014590 ENSRNOT00000017542 ENSRNOT00000016409 ENSRNOT00000045509 ENSRNOT00000034377 ENSRNOT00000055716 ENSRNOT00000071898 ENSRNOT00000002935 ENSRNOT00000073752 ENSRNOT00000050690 ENSRNOT00000041103 ENSRNOT00000031545 ENSRNOT00000047694 ENSRNOT00000039882 ENSRNOT00000041927 ENSRNOT00000047511 ENSRNOT00000047713 ENSRNOT00000045222 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   RGD1562953
Gene Name:   60S ribosomal protein L7a
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000033843
Entrez:   499382
UniGene:   Not Available
Chromosome Mapping:   1q55

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 36.320000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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