PrimePCR™ Probe Assay: Olr1470, Rat


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0038433
Assay Design:   exonic
Chromosome Location:   10:59722864-59722986question
Amplicon Length:   93
Splice Variants Targeted:   ENSRNOT00000052183 ENSRNOT00000065541 ENSRNOT00000074380 ENSRNOT00000045573 ENSRNOT00000073603 ENSRNOT00000041318 ENSRNOT00000042409 ENSRNOT00000072790 ENSRNOT00000045221 ENSRNOT00000074729 ENSRNOT00000074648 ENSRNOT00000071283 ENSRNOT00000072328 ENSRNOT00000075388 ENSRNOT00000071349 ENSRNOT00000071144 ENSRNOT00000041085 ENSRNOT00000050847 ENSRNOT00000066983 ENSRNOT00000072702 ENSRNOT00000045673

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr1470
Gene Name:   Olfactory receptor-like protein I9
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000047556
Entrez:   404975
UniGene:   Rn.129823
Chromosome Mapping:   10q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.040000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download