PrimePCR™ Probe Assay: Smcp, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0038357
Assay Design:   exonic
Chromosome Location:   2:212094713-212094850question
Amplicon Length:   108
Splice Variants Targeted:   ENSRNOT00000012371

Gene Information

cysteine-rich protein; involved in sperm motility [RGD, Feb 2006]

Gene Symbol:   Smcp
Gene Name:   Sperm mitochondrial-associated cysteine-rich protein
Aliases:   Not Available
RefSeq:   NM_031536
Ensembl:   ENSRNOG00000009326
Entrez:   24899
UniGene:   Rn.10492
Chromosome Mapping:   2q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.350000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download